Webinar: 3DEM of Brain Tissue Using Rapid Automated Preparation

  • Date

    Thursday, October 22, 2020

  • Time

    11:00 – 11:50 AM CDT

  • Speaker

    Emily Benson, BS, 3DEM Ultrastructural Imaging and Computation Core, Cleveland Clinic

  • Cost

    FREE  There is no cost to participate, but space is limited, and registration is required.

Serial block-face scanning electron microscopy (a type of 3-dimensional electron microscopy—3DEM) provides uniquely powerful morphological insight into neurological structures, such as to map and quantify synapses. While image acquisition has been automated and streamlined using computer control, sample preparation remains a tedious manual process, requiring dozens of exchanges of toxic reagents over the course of a week.

 

Emily Benson, Senior Research Technologist at the Cleveland Clinic, will present a webinar on automating an “OTO” specimen preparation protocol for 3DEM of neurological tissues. Benson will describe her sample preparation work, where she reduced a 4 to 5 day manual specimen preparation process, including 100% resin infiltration, to an automated process that required just 7.5 hours.

About the Speaker

Emily Benson, BS, is a senior research technologist at the Cleveland Clinic. She has 9 years experience as a large volume 3DEM applications specialist, working both in industry (Renovo Neural) and academic settings. She has extensive experience in serial block face imaging, image reconstruction, and data extraction. For her work on 3DEM preparation of brain tissue, she received a 2020 Microscopy & Microanalysis Travel Award, where her presentation received first place for the esteemed Diatome Award.

Takaways

Labs preparing specimens for 3DEM will learn how automated preparation:

  • Reduces specimen preparation time by >75%
  • Decreases handling and exposure to toxic reagents
  • Cuts operator hands-on effort to just 1 hour instead of 2 days
  • Prepares qualitatively and quantitatively comparable results in side-by-side comparison to manually prepared specimens
  • Provides overall laboratory efficiency

Questions about this webinar?  Contact us!