Walk-away Convenience
While you focus on other lab projects, the ASP-1000 automatically prepares your samples!
One instrument prepares TEM and 3DEM specimens and does on-grid and pre-embedding immuno-gold labeling
Complicated protocols? Simultaneously prepare titrations and controls – up to 72 reagent steps, 576 reagents, unlimited program steps
Expensive reagents? No problem. Requires only microliters
Identical processing provides comparable results every time
Efficient agitation ensures excellent mixing
Efficient agitation accelerates tissue preparation for TEM and 3DEM from days to hours:
- Renal biopsy TEM prep in 2 hours
- Muscle TEM in 133 minutes – Applications Note and Publication
- Liver TEM in 133 minutes
- Planaria TEM in 3 hours, instead of 7 days
- Tissue in LR White for TEM immuno in 45 minutes
- Agar enrobed cells for TEM in 1.5 hours
- 3DEM of brain prep in a day – First place winner 2020 Diatome Poster Award
- 3DEM of heart or brain and liver in a day
- 3DEM of planaria in 1/2 day of effort, instead of 5 days
Stop babysitting reagent exchanges!
- TEM specimen fixation, en bloc staining, dehydration, and embedding
- Immunolabeling – On grid and en bloc
- 3DEM specimen fixation, staining, dehydration, and embedding
- SEM specimen fixation and dehydration
- Easy set-up and clean-up.
Control of Bio Reaction Automation (COBRA) software
- Pre-written protocols
- Create new protocols – specify reagent, reaction time, and agitation
- Two-tiered access: Operator and Developer
- Precise and efficient protocols with greater reproducibly than humanly possible.
Download ASP-1000 Brochure
Using the ASP-1000
1. Select a protocol with the ASP COBRA software, or create a new protocol
2. Attach mPrep capsules with loaded specimens or grids
3. Place reagents in microwell plates on the ASP-1000 tray. Optionally seal reagent plates with disposable covers to cut air exposure and prevent evaporation
4. Press START. The ASP-1000 does the rest:
– mPrep capsules move from reagent to reagent
– Reagents are drawn into mPrep capsules for the specified time
– Agitation flows reagent in and out of capsules for excellent mixing
– mPrep capsules punch through optional reagent covers. Reagents are always fresh
5. When the protocol is completed, remove capsules from the ASP-1000:
– For TEM and SBEM embedding there is no messy handling of specimens in resin. Eight resin-filled mPrep/s capsules are simply transferred to the oven to cure the resin
– For grids, mPrep/g capsules are removed and stored in an mPrep Capsule Grid box. No tedious handling of grids
– For SEM preparation simply transfer capsules directly to the CPD holder with no direct specimen handling.
Product Description
Capacity
- 8 mPrep capsules for simultaneously preparing 8 specimens or 16 grids.
- Unlimited protocol steps
- Up to 72 reagent operations on the deck at a time
- Up to 576 reagents using 96-well microwell plates to hold reagents
Includes
- Base unit automation platform with proven track record for reliability
- Eight channel head
- Pump module
- Integrated removable fume enclosure
- Microwell tray (easy clean HDPE) accommodates up to 6 SBS 96-well or 12-well reservoirs
- Laptop Controller with multiple preinstalled protocols
Installation requirements
- 120-130 VAC 60 cycle standard. For other power needs please inquire
- The ASP-1000 should be installed to vent into a fume hood for safety with electron microscopy reagents
- Required space for base unit including integrated cover: 48 cm wide x 48 cm deep x 64 cm high (19 x 24 x 25 inches)
- Required space for pump module near base unit: 21 cm wide x 21 cm deep x 31 cm high (8 x 8 x 12 inch)
Download the ASP-100 brochure
Required
mPrep/g capsules to prepare TEM grids
mPrep/s capsules to prepare specimens
mPrep/f couplers to prevent introducing damaging reagents into ASP-1000
mPrep/Bench to transfer resin-filled mPrep/s capsules to oven for resin curing
Microwell plates to hold reagents. Use either 96-well or 12-well
Recommended
mPrep CPD holder when using a Tousimus critical point dryer
Microwell plate covers to cover and seal reagents in microwell plates
The ASP-1000 can be used for the full range of specimen and grid preparation applications. Please refer to TEM, SEM and 3DEM, and Materials Applications.
See how the ASP-1000 has been used for these applications:
- Preparing kidney for TEM in 45 minutes from postfix to oven curing
- Preparing core needle kidney biopsies for TEM including en bloc uranyl acid staining in 2 hours prior to resin curing
- Processing oriented mammalian muscle for TEM in 2 hours from postfix to oven curing
- Serial block face specimen preparation of brain, heart, and planeria in about one day, instead of a week
- Rapid automated preparation of Planarian worm tissue for TEM in 3 hours
- Fully automated immunogold pre-embedding and on grid labeling
- Fixation and embedding in LR White resin in 45 minutes
- Liver reliably and consistently prepared for TEM in just 133 minutes prior to resin curing
- Planaria fixed, en bloc stained and ready for for 3D SEM block face imaging in 1.5 days
- Automated preparation of nano-particle TEM specimens.
ASP-1000
ASP-1000™ mPrep Automatic Specimen Processor
Programable specimen and grid preparation. Request a Quotation
Product SKU: ASP1000
96-well Plates – 500ul per well
96-well plates, polypropylene, round wells with U-bottom, 10/sleeve
Product SKU: 51001$39.00
96-well plates, square well, 1.2ml, polypropylene,10/SLV, sold by SLEEVE
96-well plates, polypropylene, V-bottom, 10/sleeve
Product SKU: 51010$42.00
96-well plates, square well, 1.2ml, polypropylene,100/CS, sold by CASE
96-well plates, polypropylene, V-bottom, 100/case
Product SKU: 51011$349.00
12-Channel Reagent Reservoir – Single pack
12-channel reagent reservoir, polypropylene
Product SKU: 52501$7.59
For a complete listing of mPrep products, click Purchase.
- ASP 1000 product info
-
Using the ASP-1000
1. Select a protocol with the ASP COBRA software, or create a new protocol
2. Attach mPrep capsules with loaded specimens or grids
3. Place reagents in microwell plates on the ASP-1000 tray. Optionally seal reagent plates with disposable covers to cut air exposure and prevent evaporation
4. Press START. The ASP-1000 does the rest:
– mPrep capsules move from reagent to reagent
– Reagents are drawn into mPrep capsules for the specified time
– Agitation flows reagent in and out of capsules for excellent mixing
– mPrep capsules punch through optional reagent covers. Reagents are always fresh
5. When the protocol is completed, remove capsules from the ASP-1000:
– For TEM and SBEM embedding there is no messy handling of specimens in resin. Eight resin-filled mPrep/s capsules are simply transferred to the oven to cure the resin
– For grids, mPrep/g capsules are removed and stored in an mPrep Capsule Grid box. No tedious handling of grids
– For SEM preparation simply transfer capsules directly to the CPD holder with no direct specimen handling.
Product Description
Capacity
- 8 mPrep capsules for simultaneously preparing 8 specimens or 16 grids.
- Unlimited protocol steps
- Up to 72 reagent operations on the deck at a time
- Up to 576 reagents using 96-well microwell plates to hold reagents
Includes
- Base unit automation platform with proven track record for reliability
- Eight channel head
- Pump module
- Integrated removable fume enclosure
- Microwell tray (easy clean HDPE) accommodates up to 6 SBS 96-well or 12-well reservoirs
- Laptop Controller with multiple preinstalled protocols
Installation requirements
- 120-130 VAC 60 cycle standard. For other power needs please inquire
- The ASP-1000 should be installed to vent into a fume hood for safety with electron microscopy reagents
- Required space for base unit including integrated cover: 48 cm wide x 48 cm deep x 64 cm high (19 x 24 x 25 inches)
- Required space for pump module near base unit: 21 cm wide x 21 cm deep x 31 cm high (8 x 8 x 12 inch)
Download the ASP-100 brochure
- System components
-
Required
mPrep/g capsules to prepare TEM grids
mPrep/s capsules to prepare specimens
mPrep/f couplers to prevent introducing damaging reagents into ASP-1000
mPrep/Bench to transfer resin-filled mPrep/s capsules to oven for resin curing
Microwell plates to hold reagents. Use either 96-well or 12-well
Recommended
mPrep CPD holder when using a Tousimus critical point dryer
Microwell plate covers to cover and seal reagents in microwell plates
- Applications
-
The ASP-1000 can be used for the full range of specimen and grid preparation applications. Please refer to TEM, SEM and 3DEM, and Materials Applications.
See how the ASP-1000 has been used for these applications:
- Preparing kidney for TEM in 45 minutes from postfix to oven curing
- Preparing core needle kidney biopsies for TEM including en bloc uranyl acid staining in 2 hours prior to resin curing
- Processing oriented mammalian muscle for TEM in 2 hours from postfix to oven curing
- Serial block face specimen preparation of brain, heart, and planeria in about one day, instead of a week
- Rapid automated preparation of Planarian worm tissue for TEM in 3 hours
- Fully automated immunogold pre-embedding and on grid labeling
- Fixation and embedding in LR White resin in 45 minutes
- Liver reliably and consistently prepared for TEM in just 133 minutes prior to resin curing
- Planaria fixed, en bloc stained and ready for for 3D SEM block face imaging in 1.5 days
- Automated preparation of nano-particle TEM specimens.
- Purchase
-
ASP-1000
ASP-1000™ mPrep Automatic Specimen Processor
Programable specimen and grid preparation. Request a Quotation
Product SKU: ASP1000
96-well Plates – 500ul per well
96-well plates, polypropylene, round wells with U-bottom, 10/sleeve
Product SKU: 51001$39.00
96-well plates, square well, 1.2ml, polypropylene,10/SLV, sold by SLEEVE
96-well plates, polypropylene, V-bottom, 10/sleeve
Product SKU: 51010$42.00
96-well plates, square well, 1.2ml, polypropylene,100/CS, sold by CASE
96-well plates, polypropylene, V-bottom, 100/case
Product SKU: 51011$349.00
12-Channel Reagent Reservoir – Single pack
12-channel reagent reservoir, polypropylene
Product SKU: 52501$7.59
For a complete listing of mPrep products, click Purchase.