ASP™ Automated Specimen Processors

ASP™ Automated Specimen Processors

ASP™ instruments enable automated, accelerated preparation of biological samples for high-quality TEM and 3D volume EM (vEM) including serial block, focused ion beam and array tomography SEM. Two versions are available, the ASP-1000 and the ASP-2000.  The ASP-2000 has all the features of the ASP-1000 with the added benefit of temperature controlled reagents.

 

One instrument prepares TEM and vEM specimens and does on-grid and pre-embedding immuno-gold labeling.

Complicated protocols?  Simultaneously prepare titrations and controls – up to 72 reagent steps and 576 reagents. Unlimited protocol steps enable even more reagent steps for any protocol.  The ASP-2000 adds automated reagent temperature control.

Expensive reagents? No problem. Requires only microliters.

Identical processing provides comparable results every time.

Efficient agitation ensures excellent mixing.

 

mPrep ASPs accelerate TEM and vEM preparation from days to hours:

Stop babysitting reagent exchanges!

  • TEM specimen fixation, en bloc staining, dehydration, and embedding
  • Immunolabeling – On grid and pre-embedding (en bloc)
  • vEM specimen fixation, staining, dehydration, and embedding
  • SEM specimen fixation and dehydration
  • Easy set-up and clean-up.

Easy Intuitive Protocol Creation

  • Pre-written protocols for TEM and vEM
  • Create new protocols – specify reagent, reaction time, agitation, and reagent temperature (ASP-2000)
  • Two-tiered access: Operator and Developer
  • Precise and efficient protocols with greater reproducibly than humanly possible.

Download ASP-1000 Brochure

Download ASP-2000 Brochure Supplement


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Using ASP processors

1. Select a protocol (or easily create a new protocol)

2.  Attach mPrep capsules with loaded specimens or grids

3.  Place reagents in microwell plates on the ASP tray. Optionally seal reagent plates with disposable covers to cut air exposure and prevent evaporation

4.  Press START.  The ASP does the rest:

– mPrep capsules move from reagent to reagent

– Reagents are drawn into mPrep capsules for the specified time

– Agitation flows reagent in and out of capsules for excellent mixing

– mPrep capsules punch through optional reagent covers. Reagents are always fresh

5.  When the protocol is completed, remove capsules from the ASP:

– For TEM and SBEM embedding there is no messy handling of specimens in resin. Eight resin-filled mPrep/s capsules are simply transferred to the oven to cure the resin

– For grids, mPrep/g capsules are removed and stored in an mPrep Capsule Grid box. No tedious handling of grids

– For SEM preparation simply transfer capsules directly to the CPD holder with no direct specimen handling.

Product Description

Capacity

  • 8 mPrep capsules to simultaneously prepare 16 grids or 8 specimens (or up to 24-32 specimens for high throughput needs)
  • Unlimited protocol steps
  • Up to 72 reagent operations on the deck at a time
  • Up to 576 reagents using 96-well microwell plates to hold reagents

 

Includes

  • Automation platform with proven track record for reliability
  • Eight channel head
  • Pump module
  • Integrated removable fume enclosure
  • Microwell tray accommodates up to 6 standard 96-well or 12-well reservoirs
  • Laptop Controller with multiple preinstalled protocols

 

Installation requirements

  • 120-130 VAC 60 cycle standard. For other power needs please inquire
  • ASPs should be installed to vent into a fume hood for safety with electron microscopy reagents
  • Required space for base unit including integrated cover: 48 cm wide x 48 cm deep x 64 cm high (19 x 24 x 25 inches)
  • Required space for pump module near base unit: 21 cm wide x 21 cm deep x 31 cm high (8 x 8 x 12 inch)

 

Download the ASP-1000 brochure

Download the ASP-2000 brochure supplement

Required

mPrep/g capsules to prepare TEM grids

mPrep/s capsules to prepare specimens

mPrep/f couplers to prevent introducing damaging reagents into ASP

mPrep/Bench to transfer resin-filled mPrep/s capsules to oven for resin curing

Microwell plates to hold reagents. Use either 96-well or 12-well

Recommended

mPrep CPD holder for use with critical point dryers and some cryo-preparation applications

Microwell plate covers to cover and seal reagents in microwell plates 

For a complete listing of mPrep products, click Purchase.

ASP product info

Using ASP processors

1. Select a protocol (or easily create a new protocol)

2.  Attach mPrep capsules with loaded specimens or grids

3.  Place reagents in microwell plates on the ASP tray. Optionally seal reagent plates with disposable covers to cut air exposure and prevent evaporation

4.  Press START.  The ASP does the rest:

– mPrep capsules move from reagent to reagent

– Reagents are drawn into mPrep capsules for the specified time

– Agitation flows reagent in and out of capsules for excellent mixing

– mPrep capsules punch through optional reagent covers. Reagents are always fresh

5.  When the protocol is completed, remove capsules from the ASP:

– For TEM and SBEM embedding there is no messy handling of specimens in resin. Eight resin-filled mPrep/s capsules are simply transferred to the oven to cure the resin

– For grids, mPrep/g capsules are removed and stored in an mPrep Capsule Grid box. No tedious handling of grids

– For SEM preparation simply transfer capsules directly to the CPD holder with no direct specimen handling.

Product Description

Capacity

  • 8 mPrep capsules to simultaneously prepare 16 grids or 8 specimens (or up to 24-32 specimens for high throughput needs)
  • Unlimited protocol steps
  • Up to 72 reagent operations on the deck at a time
  • Up to 576 reagents using 96-well microwell plates to hold reagents

 

Includes

  • Automation platform with proven track record for reliability
  • Eight channel head
  • Pump module
  • Integrated removable fume enclosure
  • Microwell tray accommodates up to 6 standard 96-well or 12-well reservoirs
  • Laptop Controller with multiple preinstalled protocols

 

Installation requirements

  • 120-130 VAC 60 cycle standard. For other power needs please inquire
  • ASPs should be installed to vent into a fume hood for safety with electron microscopy reagents
  • Required space for base unit including integrated cover: 48 cm wide x 48 cm deep x 64 cm high (19 x 24 x 25 inches)
  • Required space for pump module near base unit: 21 cm wide x 21 cm deep x 31 cm high (8 x 8 x 12 inch)

 

Download the ASP-1000 brochure

Download the ASP-2000 brochure supplement

System components

Required

mPrep/g capsules to prepare TEM grids

mPrep/s capsules to prepare specimens

mPrep/f couplers to prevent introducing damaging reagents into ASP

mPrep/Bench to transfer resin-filled mPrep/s capsules to oven for resin curing

Microwell plates to hold reagents. Use either 96-well or 12-well

Recommended

mPrep CPD holder for use with critical point dryers and some cryo-preparation applications

Microwell plate covers to cover and seal reagents in microwell plates 

Applications
Purchase

For a complete listing of mPrep products, click Purchase.