mPrep/s Workstation

mPrep/s™ Workstation efficiently loads and orients specimens into mPrep/s capsules.  Use for TEM and SEM.

 

Accurate orientation. Specimens may be oriented using several methods

Efficient workflow. Streamlines specimen processing from dissection to reagent processing

Reduce handling. Once loaded in capsules, specimens are not touched again for TEM embedding or SEM mounting

Easy dissection. Single focal plane keep specimens in focus during dissection and when loading specimens in capsules

Wet samples. Dissect and load specimens wetted by buffers or fixatives

Efficient reagent processing. Directly load capsules onto pipettor from Workstation


Organized for efficient workflow


Product Description

  • Durable, corrosion-resistant materials for long service with saline, buffers and aldehyde fixatives. Can be fully immersed for cleaning.  Safe cleaning with detergents, bleach and alcohol
  • Top cutting surface is:
    • Soft high density polyethylene (HDPE) that minimizes dulling of fine dissection tools
    • Low glare and lightly textured surface for optimal visibility and easy specimen handling
    • Top is easily replaced if it becomes marred with cuts after long use
  • 6 dissection wells to organize and prepare specimens, and keep them wet
  • 12 capsule wells and screen storage in back holds 12 capsules until used
  • 24 capsule wells (12 per side) to hold mPrep/s capsules after loading with specimens and to keep them wet. Spaced for direct attachment of capsules onto 8 and 12-channel pipettors
  • Rounded front edge provides comfortable use
  • Feet adjust to change height. Adjust for comfort and to straddle many dissecting microscope bases
  • Precision mechanism is fully adjustable.

Using the mPrep Workstation

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Prepare the specimen

  • Trim and dissect on Workstation surface
  • Use shallow wells to keep specimens organized
  • Add water or fixative to wells during dissection

Load screen (and unoriented specimens)

  • Insert mPrep/s screen into Workstation insertion tool
  • Engage screen tabs into insertion tool flanges

Orient specimen – Back Pinch Method

  • Press Workstation lever to open screen
  • Place back end of specimen into screen opening
  • Release lever to pinch specimen
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Encapsulate specimen

  • Slide capsule down to hold specimen in place
  • For Back Pinch Orientation method leave space above specimen

Remove capsule from Workstation

  • Rotate capsule counter-clockwise to disengage screen from Workstation insertion tool
  • Lift capsule off Workstation

Set aside encapsulated specimen

  • Place capsule into a Workstation well
  • Add fluids to wells to keep specimens wet
  • Load capsules directly onto pipettor for reagent processing

Application Notes

This application note illustrates the preparation of polymer specimens for cross-sectional analysis. Polymer drug delivery particles were embedded and cross-sectioned for transmission FTIR spectroscopy and EDS-SEM. The application note also shows how to extend these methods to the preparation of other soft materials including coatings, thin films and fibers.

This application note illustrates mammalian kidney tissue prep for TEM from fixation through embedding. This general protocol applies to other specimens that require embedding for cross-sectioning. Photos show the mPrep capsule reagent exchange process and how mPrep TEM blocks are faced and sectioned while still in capsules. Supplies and the reagent formulae are listed.

This application note presents a fast, fully-automated method of tissue processing. Mammalian kidney was prepared for TEM with the mPrep ASP-1000 programmed for highly efficient rapid-agitation. Reagent processing from postfix rinse through 100% resin infiltration was accomplished in just 45 minutes.


mPrep workstation applications

Processing tissue for SEM can be tedious. There is potential for specimen damage due to contact with labware and critical point dryers. Orienting and mounting brittle CPD prepared tissues is challenging. With mPrep/s capsules specimens are protected and can be oriented prior to CPD processing.

Multiple cryogenic manipulations are required to rapidly freeze and orient tissue specimens for cryo-facing and SEM. With mPrep/s capsules, tissue samples are oriented prior to freezing and secured in the capsule through cryo-facing and SEM mounting.

Embedding and orienting polymers for cross sectioning is challenging: Particles are difficult to entrap and orienting fibers and films is time consuming. With mPrep/s capsules particles are easily entrapped, and fibers or films are oriented in seconds using the mPrep Workstation. Once loaded in mPrep/s capsules, multiple specimens can be simultaneously embedded.


Videos

Using the mPrep Workstation for Oriented and Unoriented Specimens

The tutorial starts by introducing the mPrep Workstation. Then it demonstrates how to load and orient specimens into mPrep capsules. Three methods are shown: pinch, compression and cup. 5 minutes 19 seconds

Webinar: TEM & SEM Sample Preparation with mPrep Capsules

This webinar introduces and demonstrates the mPrep System.  Webinar covers how the mPrep System works. It discusses the benefits of the mPrep System over current practice. And it addresses multiple TEM and SEM applications: tissues,  macromolecules, immuno, nanoparticles, cryo-planing. 45 minutes


Instructions

How to use the mPrep CPD holder for specimen and grid sample preparation using the critical point method.

Instructions for using mPrep/s capsules for specimen handling and processing.

A single page graphical display of the three steps to orient and load specimens into mPrep/s capsules. Both compression and pinch methods are shown. Use this guide as a lab reference poster.


mPrep/s workstation products

mPrep/s™ Capsule Pack

8 capsules, 12 screens, storage box, labels

Product SKU: 22200

$48.00

mPrep/s™ Capsules – 96 count

96 capsules, screens & blank label sets

Product SKU: 22500

$576.00

mPrep/s™ Workstation

Prepare specimens and insert into mPrep/s capsules with or without orientation using integrated screen insertion tool

Product SKU: 42100

$950.00

mPrep/f 30 Standard Filter Coupler™

16 couplers

Product SKU: 31500

$48.00

For a complete listing of mPrep products, click Purchase.