mPrep/s™ Workstation efficiently loads and orients specimens into mPrep/s capsules. Use for TEM and SEM.
Accurate orientation. Specimens may be oriented using several methods
Efficient workflow. Streamlines specimen processing from dissection to reagent processing
Reduce handling. Once loaded in capsules, specimens are not touched again for TEM embedding or SEM mounting
Easy dissection. Single focal plane keep specimens in focus during dissection and when loading specimens in capsules
Wet samples. Dissect and load specimens wetted by buffers or fixatives
Efficient reagent processing. Directly load capsules onto pipettor from Workstation
This application note illustrates the preparation of polymer specimens for cross-sectional analysis. Polymer drug delivery particles were embedded and cross-sectioned for transmission FTIR spectroscopy and EDS-SEM. The application note also shows how to extend these methods to the preparation of other soft materials including coatings, thin films and fibers.
This application note illustrates mammalian kidney tissue prep for TEM from fixation through embedding. This general protocol applies to other specimens that require embedding for cross-sectioning. Photos show the mPrep capsule reagent exchange process and how mPrep TEM blocks are faced and sectioned while still in capsules. Supplies and the reagent formulae are listed.
This application note presents a fast, fully-automated method of tissue processing. Mammalian kidney was prepared for TEM with the mPrep ASP-1000 programmed for highly efficient rapid-agitation. Reagent processing from postfix rinse through 100% resin infiltration was accomplished in just 45 minutes.
This application note presents a method for processing mammalian muscle tissue for TEM in just over two hours. Using the ASP-1000 automated specimen processor, specimens were processed from postfixation rinse through infiltration in just 133 minutes. Specimens were handled only once, when they were oriented and inserted into mPrep/s capsules using the mPrep/s Workstation.
Processing tissue for SEM can be tedious. There is potential for specimen damage due to contact with labware and critical point dryers. Orienting and mounting brittle CPD prepared tissues is challenging. With mPrep/s capsules specimens are protected and can be oriented prior to CPD processing.
Multiple cryogenic manipulations are required to rapidly freeze and orient tissue specimens for cryo-facing and SEM. With mPrep/s capsules, tissue samples are oriented prior to freezing and secured in the capsule through cryo-facing and SEM mounting.
Embedding and orienting polymers for cross sectioning is challenging: Particles are difficult to entrap and orienting fibers and films is time consuming. With mPrep/s capsules particles are easily entrapped, and fibers or films are oriented in seconds using the mPrep Workstation. Once loaded in mPrep/s capsules, multiple specimens can be simultaneously embedded.
How to use the mPrep CPD holder for specimen and grid sample preparation using the critical point method.
Instructions for using mPrep/s capsules for specimen handling and processing.
A single page graphical display of the three steps to orient and load specimens into mPrep/s capsules. Both compression and pinch methods are shown. Use this guide as a lab reference poster.