16 Jul Bio tissue cryo-facing
The mPrep system makes cryo-facing easy!
Problems
- Morphological preservation requires small specimens that are easily lost in liquid nitrogen (LN2)
- Orienting specimens immersed in LN2 is difficult
- Cryo-cutting (facing) requires custom engineering, or is manual and less accurate
- Orientation may be lost during transfer to SEM
- Specimens are damaged or lost due to multiple handling steps
mPrep solutions
- mPrep capsules hold small tissue specimens throughout sample preparation
- The mPrep Workstation accurately orients specimens in seconds
- mPrep capsules fit onto standard cryo-microtomes for all cryo-facing operations
- mPrep/s screens maintain tissue orientation throughout processing
- mPrep capsules protect specimens from loss or damage
Cactus (SEM cryo-faced)
Kidney (SEM cryo-faced)
Required
mPrep/s capsules to hold specimens
mPrep/s Workstation (w/integrated insertion tool): required to orient specimens using the pinch method. Additional benefits are:
- Precise specimen orientation in seconds
- Wet samples easy to handle
- Single focal plane keeps specimens in focus
- Simultaneous loading of capsules onto pipettor
Recommended
Reagent reservoirs
Microwell plates
mPrep/bench to seal capsule bottoms during long reagent treatments
Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules
Gilson Pipetman Neo (P200) pipettors bundled with mPrep/s capsules
mPrep/s™ Pipettor Kit single-channel
- Single-channel pipettor
- mPrep/s capsule pack
- 96 pipette tips
mPrep/s™ Pipettor Kit eight-channel
- Eight-channel pipettor
- mPrep/s capsule pack
- 96 pipette tips
Videos
This video tutorial demonstrates how to use mPrep capsules with multi-channel pipettors and common lab ware to simultaneously deliver reagents to multiple specimens and grids. The video demonstrates TEM fixation and embedding. Reagent processing procedures are similar for all specimen types and for grids with mPrep capsules .
6 minutes 4 seconds
The tutorial starts by introducing the mPrep Workstation. Then it demonstrates how to load and orient specimens into mPrep capsules. Three methods are shown: pinch, compression and cup.
5 minutes 19 seconds
This webinar introduces and demonstrates the mPrep System. Webinar covers how the mPrep System works. It discusses the benefits of the mPrep System over current practice. And it addresses multiple TEM and SEM applications: tissues, macromolecules, immuno, nanoparticles, cryo-planing.
45 minutes
mPrep specimen products
mPrep/s™ Capsule Bodies without Screens – 96 count
96 capsule bodies without screens
Product SKU: 22550$395.00
mPrep/s™ Workstation
Prepare specimens and insert into mPrep/s capsules with or without orientation using integrated screen insertion tool
Product SKU: 42100$980.00
mPrep/s™ Insertion Tool
Inserts, removes and adjusts screens in mPrep/s capsules
Product SKU: 32010$33.00
mPrep™ CPD Holder
mPrep™ CPD Holder for critical-point dry processing of up to 6 mPrep/s or 6 mPrep/g capsules. For use with Tousimis® CPD apparatus, and others with 1.25” (32 mm) diameter chambers. Also used for cryogenic specimen preparation.
Product SKU: 33010$260.00
96-well Plates – 500ul per well
96-well plates, polypropylene, round wells with U-bottom, 10/sleeve
Product SKU: 51001$43.00
96-well plates, square well, 1.2ml, polypropylene,10/SLV, sold by SLEEVE
96-well plates, polypropylene, V-bottom, 10/sleeve
Product SKU: 51010$52.00
96-well plates, square well, 1.2ml, polypropylene,100/CS, sold by CASE
96-well plates, polypropylene, V-bottom, 100/case
Product SKU: 51011$419.00
12-Channel Reagent Reservoir – Single pack
12-channel reagent reservoir, polypropylene
Product SKU: 52501$9.75