16 Aug AN 604_AgarEmbeddedCellsForTEM
Demonstrates using agar embedding of suspended cells to enable rapid automated preparation with the ASP-1000 Automated processor to fix, post-fix and embed cells in epoxy for TEM. The example uses yeast, but this method can be readily extended to the preparation of other types of cells in suspension, and for the preparation of other small specimens. By combining agar embedment with ASP-1000 processing, 18 centrifugation and resuspension cycles were eliminated from a typical manual protocol. Once the cells were agar embedded, the total processing time prior to epoxy resin curing was just 1.5 hours.