04 Mar AN605 Automated Pre-Embedding Immunogold Labeling for TEM
Automated pre-embedding (en bloc) immunogold labeling of brain using ultra-small gold and silver enhancement followed by conventional epoxy embedding. The automated workflow yielded reproducible labeling results with signal-to-noise ratio and labeling efficiency comparable to manual labeling, yet required only ~1 hr of effort, compared to 8-16 hrs effort when done manually.