Tech Note – Automated Immunogold Labeling & Gold Fiducial Particles

Tech Note – Automated Immunogold Labeling & Gold Fiducial Particles

Illustrates automation of immunogold labeling using the mPrep ASP-1000 automated specimen processor. Researchers at the University of Wisconsin prepared C. elegans tissue sections on TEM grids and then used the ASP-1000 to immunolabel an endosomal sorting component with 6 nm gold. The entire 7.5 hour immunolabeling protocol was automatically accomplished.  This note also illustrates automated application of 10 nm gold fiducial markers onto thick sections for tomography.

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