AN503 Preparing Macromolecular Specimens for TEM: Negative Staining of DNA-RecA Complex

AN503 Preparing Macromolecular Specimens for TEM: Negative Staining of DNA-RecA Complex

Illustrates the preparation of macromolecular specimens for negative stain TEM using mPrep/g capsules, pipettors and Formvar®-filmed TEM grids. The example is a DNA-RecA complex, although the protocol is applicable to many other specimens. The specimen preparation and microscopy was performed in the Department of Biochemistry at the University of Wisconsin – Madison by Mr. Jay Campbell and Dr. Sindhu Chitteni-Pattu, in the laboratory of Dr. Michael M Cox.

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