Materials applications

The mPrep System™ streamlines materials sample preparation. Use mPrep/s capsules to prepare specimens for TEM, SEM, and other analytical instruments that require sample sectioning. Use mPrep/g capsules to prepare specimens on TEM grids. mPrep System benefits include:

  • Reduced labor
  • Simultaneous reagent processing
  • Consistent quality and reproducibility
  • Reduced errors and accidents due to limited sample touches
  • Labels and document every sample every time

Nanoparticles (TEM)

Materials applications

  • Embed and cross-section polymers, soft material, films and fibers
  • Entrap small particles for easy handling
  • Orient films and fibers for SEM preparation and imaging
  • Prepare nanoparticles on grids

The mPrep System is designed for versatility. Our customers continue to develop new applications.  Explore some Materials applications below.


Hydrogel polymer sectioning



Materials applications

Preparing nanoparticles on TEM grids

The mPrep system simplifies the preparation of nanoparticles for TEM.

Problems

Nanoparticle specimen prep requires  forceps to transfer filmed grids through multiple reagents. Grids are stored in grid boxes until TEM imaging.

  • Grids and support films are easily damaged
  • Grids are easily lost or mixed up
  • Limited quantities of specimen solutions may be available
  • Specimen and reagent solutions may evaporate, precipitate or be contaminated
  • Adsorption and staining times may be inconsistent across multiple grids

 

mPrep solutions

  • Encapsulated grids reduce handling and damage
  • Grids in mPrep/g capsules are always labeled
  • Capsules require only 35 μl to prepare two grids
  • Enclosed capsules reduce or eliminate air exposure
  • Simultaneous processing assures uniform timing

Nanoparticles (TEM negative stain)

Required

mPrep/g capsules to hold TEM grids
mPrep/f couplers to prevent introducing damaging reagents into pipettors
Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules

Recommended

Reagent reservoirs

Microwell plates

mPrep/bench to seal capsule bottoms during long reagent treatments

Gilson Pipetman Neo (P200) pipettors bundled with mPrep/g capsules

mPrep/g Pipettor Kit single-channel

  • Single-channel pipettor
  • mPrep/s capsule pack
  • 96 pipette tips

mPrep/g Pipettor Kit eight-channel

  • Eight-channel pipettor
  • mPrep/g capsule pack
  • 96 pipette tips

mPrep grid products

mPrep/g™ Capsules – 16 pack

16 capsules

Product SKU: 21305

$54.00

mPrep/g™ Capsules – 96 count

96 capsules

Product SKU: 21505

$302.00

mPrep/f 30™ Standard Filter Coupler

16 filter couplers per pack

Product SKU: 31500

$54.00

mPrep/Bench™ Silicone Rack

96-well silicone rack for mPrep capsules

Product SKU: 34000

$105.00

mPrep™ CPD Holder

mPrep™ CPD Holder for critical-point dry processing of up to 6 mPrep/s or 6 mPrep/g capsules. For use with Tousimis® CPD apparatus, and others with 1.25” (32 mm) diameter chambers.  Also used for cryogenic specimen preparation.

Product SKU: 33010

$260.00

96-well Plates – 500ul per well

96-well plates, polypropylene, round wells with U-bottom, 10/sleeve

Product SKU: 51001

$43.00

12-Channel Reagent Reservoir – Single pack

12-channel reagent reservoir, polypropylene

Product SKU: 52501

$9.75

Polymer cross sections

The mPrep System streamlines sample preparation of polymers and soft materials that are embedded and cross-sectioned for analysis with SEM-EDS (energy dispersive spectroscopy), optical spectroscopies (e.g. FTIR and Raman), and TEM.

Problems

The processes to embed and cross-section polymers and soft materials are conducted using laboratory equipment that are not well suited for this purpose. Microscopists experience multiple challenges;

  • Difficultly preparing many specimens at once
  • Orienting fibers and films is time consuming
  • Difficultly handling particulate specimens
  • Specimens may be damaged during preparation
  • Keeping track of multiple specimens and protocols is difficult

 

mPrep solutions

mPrep/s capsule sample preparation addresses these challenges:

  • Simultaneous processing enables easy preparation of multiple specimens
  • Accurate orientation in seconds using the mPrep Workstation
  • Particulate specimens are entrapped throughout embedding
  • Encapsulated specimen reduces handling and damage
  • Labeled capsules are easy to track

Whole particle (SEM) and 4 EDS cross-section maps

FTIR maps polymer cross-sections

Required

mPrep/s capsules to hold specimens

An insertion tool is required to insert, remove and adjust the mPrep/s screen. There are two options:

  •  mPrep/s Insertion tool
  •  mPrep/s Workstation (w/integrated insertion tool): required to orient specimens using the pinch method. Recommended because of its additional benefits
      • Precise specimen orientation in seconds
      • Wet samples easy to handle
      • Single focal plane keeps specimens in focus
      • Simultaneous loading of capsules onto pipettor for reagent processing

    mPrep/f couplers to prevent introducing damaging reagents into pipettors

    Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules

    mPrep/bench to seal capsule bottoms during long reagent treatments and resin curing

Recommended

Reagent reservoirs

Microwell plates

Gilson Pipetman Neo (P200) pipettors bundled with mPrep/s capsules

mPrep/s™ Pipettor Kit single-channel

  • Single-channel pipettor
  • mPrep/s capsule pack
  • 96 pipette tips

mPrep/s™ Pipettor Kit eight-channel

  • Eight-channel pipettor
  • mPrep/s capsule pack
  • 96 pipette tips

Publications


Videos

Preparing Embedded TEM Specimens for Sectioning

This video tutorial shows how to trim and face an embedded block inside an mPrep/s capsule. The capsule is clamped into a microtome chuck for trimming, facing and sectioning.

1 minutes 17 seconds

Specimen and Grid Reagent Processing for TEM and SEM

This video tutorial demonstrates how to use mPrep capsules with multi-channel pipettors and common lab ware to simultaneously deliver reagents to multiple specimens and grids. The video demonstrates TEM fixation and embedding. Reagent processing procedures are similar for all specimen types and for grids with mPrep capsules .

6 minutes 4 seconds

Using the mPrep Workstation for Oriented and Unoriented Specimens

The tutorial starts by introducing the mPrep Workstation. Then it demonstrates how to load and orient specimens into mPrep capsules. Three methods are shown: pinch, compression and cup.

5 minutes 19 seconds



mPrep specimen products

mPrep/s™ Capsules – 24 pack

24 capsules, 24 screens

Product SKU: 22405

$160.00

mPrep/s™ Capsules – 96 count

96 capsules, 96 screens

Product SKU: 22505

$528.00

mPrep/s™ Capsule Bodies without Screens – 96 count

96 capsule bodies without screens

Product SKU: 22550

$395.00

mPrep/s™ Workstation

Prepare specimens and insert into mPrep/s capsules with or without orientation using integrated screen insertion tool

Product SKU: 42100

$980.00

mPrep/s™ Insertion Tool

Inserts, removes and adjusts screens in mPrep/s capsules

Product SKU: 32010

$33.00

mPrep/f 30™ Standard Filter Coupler

16 filter couplers per pack

Product SKU: 31500

$54.00

mPrep/Bench™ Silicone Rack

96-well silicone rack for mPrep capsules

Product SKU: 34000

$105.00

mPrep™ CPD Holder

mPrep™ CPD Holder for critical-point dry processing of up to 6 mPrep/s or 6 mPrep/g capsules. For use with Tousimis® CPD apparatus, and others with 1.25” (32 mm) diameter chambers.  Also used for cryogenic specimen preparation.

Product SKU: 33010

$260.00

96-well Plates – 500ul per well

96-well plates, polypropylene, round wells with U-bottom, 10/sleeve

Product SKU: 51001

$43.00

96-well plates, square well, 1.2ml, polypropylene,10/SLV, sold by SLEEVE

96-well plates, polypropylene, V-bottom, 10/sleeve

Product SKU: 51010

$52.00

96-well plates, square well, 1.2ml, polypropylene,100/CS, sold by CASE

96-well plates, polypropylene, V-bottom, 100/case

Product SKU: 51011

$419.00

12-Channel Reagent Reservoir – Single pack

12-channel reagent reservoir, polypropylene

Product SKU: 52501

$9.75