07 Jul Negative staining of macromolecules
The mPrep system simplifies the preparation of macromolecular and particulate specimens for TEM.
Problems
Negative stain specimen preparation requires fine forceps to transfer filmed grids across droplets of macromolecule or particle suspensions, negative stains, and rinses. This presents microscopists with multiple challenges:
- Grids and support films are easily damaged
- Handling grids is tedious
- Grids are easily lost or mixed up
- Limited quantities of specimen solutions may be available
- Specimen and reagent solutions may evaporate, precipitate or be contaminated
- Inconsistent adsorption and staining times across multiple grids
mPrep solutions
- Encapsulated grids reduce handling and damage
- Grids with specimens may require direct handling only at the microscope
- Grids in mPrep/g capsules are always labeled
- Capsules require only 35 μl to prepare two grids
- Enclosed capsules reduce or eliminate air exposure
- Simultaneous processing assures uniform timing
DNA RecA (TEM negative stain)
Required
mPrep/g capsules to hold TEM grids
mPrep/f couplers to prevent introducing damaging reagents into pipettors
Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules
Recommended
Reagent reservoirs
Microwell plates
mPrep/bench to seal capsule bottoms during long reagent treatments
Gilson Pipetman Neo (P200) pipettors bundled with mPrep/g capsules.
mPrep/g Pipettor Kit single-channel
- Single-channel pipettor
- mPrep/s capsule pack
- 96 pipette tips
mPrep/g Pipettor Kit eight-channel
- Eight-channel pipettor
- mPrep/g capsule pack
- 96 pipette tips
Product Descriptions
Videos
This video tutorial demonstrates how to use mPrep capsules with multi-channel pipettors and common lab ware to simultaneously deliver reagents to multiple specimens and grids. The video demonstrates TEM fixation and embedding. Reagent processing procedures are similar for all specimen types and for grids with mPrep capsules .
6 minutes 4 seconds
Product Descriptions
mPrep grid products
mPrep™ CPD Holder
mPrep™ CPD Holder for critical-point dry processing of up to 6 mPrep/s or 6 mPrep/g capsules. For use with Tousimis® CPD apparatus, and others with 1.25” (32 mm) diameter chambers. Also used for cryogenic specimen preparation.
Product SKU: 33010$260.00
96-well Plates – 500ul per well
96-well plates, polypropylene, round wells with U-bottom, 10/sleeve
Product SKU: 51001$43.00
12-Channel Reagent Reservoir – Single pack
12-channel reagent reservoir, polypropylene
Product SKU: 52501$9.75