Problems

Virus sample preparation for TEM requires using fine forceps to transfer filmed grids throughout the process.  Grids boxes may require decontamination prior to transfer to the TEM.

• Grids and support films are easily damaged
• Grids are easily lost or mixed up
• Sharp forceps are a puncture risk to protective clothing
• Grid handling is especially difficult wearing BSL3/4 positive pressure suits
• Limited quantities of specimen solutions may be available
• Specimen and reagent solutions exposed to air may evaporate, precipitate or be contaminated
• Grids and grid storage containers require decontamination
• Adsorption and staining times may be inconsistent across multiple grids

mPrep solutions

• Encapsulated grids reduce handling and damage
• Grids in mPrep/g capsules are always labeled
• Forceps are not used for virus preparation
• Direct handling of grids may require handling only at the microscope
• Capsules require only 35 μl to prepare two grids
• Enclosed capsules reduce or eliminate air exposure
• Grids in capsules are decontaminated as a unit
• Simultaneous processing assures uniform timing