16 Jul Preparing viruses on TEM grids
The mPrep system facilitates preparation of viruses and other microorganisms for TEM examination, including those prepared in Biological Safety Level 3 or 4 laboratory suites.
Problems
Virus sample preparation for TEM requires using fine forceps to transfer filmed grids throughout the process. Grids boxes may require decontamination prior to transfer to the TEM.
- Grids and support films are easily damaged
- Grids are easily lost or mixed up
- Sharp forceps are a puncture risk to protective clothing
- Grid handling is especially difficult wearing BSL3/4 positive pressure suits
- Limited quantities of specimen solutions may be available
- Specimen and reagent solutions exposed to air may evaporate, precipitate or be contaminated
- Grids and grid storage containers require decontamination
- Adsorption and staining times may be inconsistent across multiple grids
mPrep solutions
- Encapsulated grids reduce handling and damage
- Grids in mPrep/g capsules are always labeled
- Forceps are not used for virus preparation
- Direct handling of grids may require handling only at the microscope
- Capsules require only 35 μl to prepare two grids
- Enclosed capsules reduce or eliminate air exposure
- Grids in capsules are decontaminated as a unit
- Simultaneous processing assures uniform timing
T6 Phage (negative stain)
Ebola virus (negative stain)
Application Notes
Required
mPrep/g capsules to hold TEM grids
mPrep/f 13 extreme couplers to prevent introducing damaging reagents or viruses into pipettors
Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules
Recommended
Reagent reservoirs
Microwell plates
mPrep/bench to seal capsule bottoms during long reagent treatments
Gilson Pipetman Neo (P200) pipettors bundled with mPrep/g capsules.
mPrep/g Pipettor Kit single-channel
- Single-channel pipettor
- mPrep/s capsule pack
- 96 pipette tips
mPrep/g Pipettor Kit eight-channel
- Eight-channel pipettor
- mPrep/g capsule pack
- 96 pipette tips
Product Descriptions
Product Descriptions
mPrep grid products
mPrep™ CPD Holder
mPrep™ CPD Holder for critical-point dry processing of up to 6 mPrep/s or 6 mPrep/g capsules. For use with Tousimis® CPD apparatus, and others with 1.25” (32 mm) diameter chambers. Also used for cryogenic specimen preparation.
Product SKU: 33010$248.00
96-well Plates – 500ul per well
96-well plates, polypropylene, round wells with U-bottom, 10/sleeve
Product SKU: 51001$41.50
12-Channel Reagent Reservoir – Single pack
12-channel reagent reservoir, polypropylene
Product SKU: 52501$9.50