07 Jul Staining tissue sections on TEM grids

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The mPrep System streamlines the entire tissue preparation and grid staining workflow.

Problems

Staining tissue sections on grids requires using fine forceps to transfer grids into grid boxes, onto stain droplets or staining apparatuses, back into grid boxes, and eventually onto the TEM stage. As a result, microscopists experience multiple challenges:

Grids are easily damaged
Handling grids is tedious
Grids are easily lost or mixed up
Difficulty achieving uniform staining times across multiple grids
Stains exposed to air can be contaminated, precipitate, and evaporate

mPrep solutions

Encapsulated grids reduce handling and damage.
Grids may only be directly handled at the microtome and the microscope
Grids in mPrep/g capsules are always labeled
Simultaneous processing assures uniform timing
Enclosed capsules reduce and can even eliminate air exposure

Learn more about staining tissue sections on TEM grids in the application notes below.

Heart (TEM)

Brain - Vampire bat (TEM)

Application Notes

Required

mPrep/g capsules to hold TEM grids

mPrep/f couplers to prevent introducing damaging reagents into pipettors

Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules

Product Descriptions

mPrep/g Capsules product description

ASP-1000 Brochure

Publications

Capsule-Based Processing and Handling of Electron Microscopy Specimens and Grids (Microscopy Today 2015)

Journal publication

Improving the Reliability, Ease, and Efficiency of Section Staining in a Diagnostic Laboratory with the mPrep/g System for TEM Grid Processing (Microscopy & Microanalysis 2015)

Abstract

Automated, Programmable Processing of Specimens and Grids with the mPrep ASP-1000 (Microscopy & Microanalysis 2015)

Abstract

Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules (Microscopy & Microanalysis 2015)

Abstract

Reducing Reagent Consumption and Improving Efficiency of Specimen Fixation and Embedding, Grid Staining and Archiving using mPrep™ Capsule Processing (Microscopy & Microanalysis 2011)

Abstract

Videos

Specimen and Grid Reagent Processing for TEM and SEM

This video tutorial demonstrates how to use mPrep capsules with multi-channel pipettors and common lab ware to simultaneously deliver reagents to multiple specimens and grids. The video demonstrates TEM fixation and embedding. Reagent processing procedures are similar for all specimen types and for grids with mPrep capsules .

6 minutes 4 seconds

Instructions

Protocol – Staining Grids with mPrep/g Capsules

Instructions for using mPrep/g capsules for staining sections on grids with uranyl acetate and lead citrate.

mPrep/g Instructions

Instructions for using mPrep/g capsules for grid handling and processing.

(Tech note) Sample tracking, barcoding and labeling with m/Prep system

This technical note introduces using mPrep capsule labeling for tracking specimens and grids with a data management system. Topics addressed include mPrep labels, 2D datamatrix barcodes, barcode readers and laboratory information management systems.

Quick Guide mPrep/g

A graphical display illustrating how to load grids into mPrep/g capsules, and how to use pipettors to stain or label grids. Use this guide as a lab reference poster.