16 Jul Preparing nanoparticles on TEM grids
The mPrep system simplifies the preparation of nanoparticles for TEM.
Problems
Nanoparticle specimen prep requires forceps to transfer filmed grids through multiple reagents. Grids are stored in grid boxes until TEM imaging.
- Grids and support films are easily damaged
- Grids are easily lost or mixed up
- Limited quantities of specimen solutions may be available
- Specimen and reagent solutions may evaporate, precipitate or be contaminated
- Adsorption and staining times may be inconsistent across multiple grids
mPrep solutions
- Encapsulated grids reduce handling and damage
- Grids in mPrep/g capsules are always labeled
- Capsules require only 35 μl to prepare two grids
- Enclosed capsules reduce or eliminate air exposure
- Simultaneous processing assures uniform timing
Nanoparticles (TEM negative stain)
Required
mPrep/g capsules to hold TEM grids
mPrep/f couplers to prevent introducing damaging reagents into pipettors
Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules
Recommended
Reagent reservoirs
Microwell plates
mPrep/bench to seal capsule bottoms during long reagent treatments
Gilson Pipetman Neo (P200) pipettors bundled with mPrep/g capsules
mPrep/g Pipettor Kit single-channel
- Single-channel pipettor
- mPrep/s capsule pack
- 96 pipette tips
mPrep/g Pipettor Kit eight-channel
- Eight-channel pipettor
- mPrep/g capsule pack
- 96 pipette tips
mPrep grid products
mPrep™ CPD Holder
mPrep™ CPD Holder for critical-point dry processing of up to 6 mPrep/s or 6 mPrep/g capsules. For use with Tousimis® CPD apparatus, and others with 1.25” (32 mm) diameter chambers. Also used for cryogenic specimen preparation.
Product SKU: 33010$248.00
96-well Plates – 500ul per well
96-well plates, polypropylene, round wells with U-bottom, 10/sleeve
Product SKU: 51001$41.50
12-Channel Reagent Reservoir – Single pack
12-channel reagent reservoir, polypropylene
Product SKU: 52501$9.50