Problems

Immunolabeling tissue sections on TEM grids using “droplet” methods requires the timed transfer of multiple grids through a dozen reagents in a process that can take hours or even a full day. As a result, microscopists experience multiple challenges:

• Difficultly achieving consistent reagent timing, which limits experimental reproducibility
• Grids are easily damaged, lost, or mixed up
• IGL reagents exposed to air can evaporate, precipitate or become contaminated
• IGL reagents and antibodies are expensive

mPrep solutions

• The mPrep System addresses these challenges:
  • Simultaneous processing assures identical reagent timing and reproducible results
  • Encapsulated grids are identified and protected from loss or damage
  • Enclosed capsules limit air exposure
  • mPrep/g capsules require only 20 ul per grid

Automated IGL with the mPrep ASP-1000

With the ASP-1000 the entire immunolabeling process is automated. Simply set up the reagents, start the program, and walk away for the rest of the day or overnight. Use with mPrep/g capsules for grids, and mPrep/s capsules for tissue en bloc IGL.

Manual IGL with a multi-channel pipettor

Using a multichannel pipettor ensures that reagent incubation timing is identical for all grids. Because grids are safely encapsulated reagent exchanges are fast and easy. Use with mPrep/s capsules for tissue en bloc IGL.