Transmission electron microscopy (TEM)

The mPrep System™ streamlines Transmission Electron Microscopy (TEM) sample preparation using a capsule based approach. Use mPrep/s capsules to fix, orient, embed, and section specimens. Use mPrep/g capsules to stain or immuno-label TEM grids.

 

The mPrep System efficiently produces quality results from every sample.  Imagine this in your lab.

  • As few as two human touches from microtome to microscope – reduces damage and loss
  • Grids and capsules labeled for easy tracking from start to storage
  • Capsules attach to common lab pipettors for controlled reagent timing and minimal reagent consumption
  • Parallel processing
    • Stain from one to dozens of grids simultaneously using multi-channel pipettors
    • Automate using the mPrep-ASP-1000 Automated Specimen Processor
    • Identical reagent timing
    • Reduced tedium and labor costs

 

The mPrep System is designed for versatility. Our customers continue to develop new applications. Explore some TEM applications below.

Kidney (TEM)

Nanoparticles (TEM negative stain)

TEM applications

Biological tissue fixation, embedding and orientation

The mPrep System streamlines the entire tissue preparation and grid staining workflow.

Problems

Tissue prep for TEM can require dozens of reagent exchanges per specimen, using laboratory equipment not well suited for this purpose.  This presents microscopists with multiple challenges:

  • Difficulty achieving uniform processing times across multiple samples
  • Reagent consumption excessive relative to the amount required to ensure reaction
  • Achieving proper orientation is time consuming
  • Specimens damage from contact with air, vials, and transfer pipettes
  • Lost specimens when difficult to see or they stick to containers

 

mPrep Solutions

  • Automated walk-away reagent processing with the mPrep ASP-1000
  • Simultaneous processing of reagent exchanges assures uniform timing
  • Controlled reagent usage with pipettors = reduced reagent consumption
  • Accurate orientation in seconds using the mPrep Workstation
  • Encapsulated specimen reduces handling = fewer damaged specimens
  • Entrapped specimens are harder to lose. Will not float.
  • Labeled capsules are easy to track.

Cardiac muscle (TEM)

Skin (TEM)

Application Notes

This application note demonstrates the reproducibility of rapid processing with the ASP-1000. Twenty-four (24) specimens from the same aldehyde perfusion fixed liver were prepared for TEM. Specimens were directly handled only once, when they were orientated in mPrep/s capsules. Automated processing required only 133 minutes from post-fix rinse through osmium fixation and 100% resin infiltration. All 24 samples showed well-preserved ultrastructure homogeneity as verified by TEM imaging.

This application note illustrates mammalian kidney tissue prep for TEM from fixation through embedding. This general protocol applies to other specimens that require embedding for cross-sectioning. Photos show the mPrep capsule reagent exchange process and how mPrep TEM blocks are faced and sectioned while still in capsules. Supplies and the reagent formulae are listed.

This application note demonstrates using agar embedding of suspended cells to enable rapid automated preparation with the ASP-1000 Automated processor to fix, post-fix and embed cells in epoxy for TEM.  The example uses yeast, but this method can be readily extended to the preparation of other types of cells in suspension, and for the preparation of other small specimens. By combining agar embedment with ASP-1000 processing, 18 centrifugation and resuspension cycles were eliminated from a typical manual protocol. Once the cells were agar embedded, the total processing time prior to epoxy resin curing was just 1.5 hours.

This application note presents a method for processing mammalian muscle tissue for TEM in just over two hours. Using the ASP-1000 automated specimen processor, specimens were processed from postfixation rinse through infiltration in just 133 minutes. Specimens were handled only once, when they were oriented and inserted into mPrep/s capsules using the mPrep/s Workstation.

This application note presents a fast, fully-automated method of tissue processing. Mammalian kidney was prepared for TEM with the mPrep ASP-1000 programmed for highly efficient rapid-agitation. Reagent processing from postfix rinse through 100% resin infiltration was accomplished in just 45 minutes.

Required

mPrep/s capsules to hold specimens

An insertion tool is required to insert, remove and adjust the mPrep/s screen. There are two options:

  •  mPrep/s Insertion tool
  •  mPrep/s Workstation (w/integrated insertion tool): required to orient specimens using the pinch method. Recommended because of its additional benefits
    • Precise specimen orientation in seconds
    • Wet samples easy to handle
    • Single focal plane keeps specimens in focus
    • Simultaneous loading of capsules onto pipettor for reagent processing

mPrep/f couplers to prevent introducing damaging reagents into pipettors

Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules

mPrep/bench to seal capsule bottoms during long reagent treatments and resin curing

Recommended

Reagent reservoirs

Microwell plates

Gilson Pipetman Neo (P200) pipettors bundled with mPrep/s capsules

mPrep/s™ Pipettor Kit single-channel

  • Single-channel pipettor
  • mPrep/s capsule pack
  • 96 pipette tips

mPrep/s™ Pipettor Kit eight-channel

  • Eight-channel pipettor
  • mPrep/s capsule pack
  • 96 pipette tips

Publications

Rapid Automated en Bloc Staining for SEM of Sections (Microscopy & Microanalysis 2017) 

Reducing Reagent Consumption and Improving Efficiency of Specimen Fixation and Embedding, Grid Staining and Archiving using mPrep™ Capsule Processing (Microscopy & Microanalysis 2011) 

High Throughput Multi Parameter TEM Chemical Processing Protocol Development with the mPrep-s Capsule System: Schmidtea mediterranea (Microscopy & Microanalysis 2014)

Sex-Specific Skeletal Muscle Fatigability and Decreased Mitochondrial Oxidative Capacity in Adult Rats Exposed to Postnatal Hyperoxia (Frontiers Physiology 2018)  

Bringing Speed and Automation Together with the ASP1000 (Microscopy & Microanalysis 2016) 

Capsule-Based Processing and Handling of Electron Microscopy Specimens and Grids (Microscopy Today 2015) 

Automated Preparation of Core Needle Biopsy Specimens for TEM Imaging (Microscopy & Microanalysis 2019)

Preparing LR White Embedded Tissue with mPrep/s Specimen Capsules (Microscopy & Microanalysis 2017) 

Fast, Walk-away, Automated Processing of Mammalian Tissue for LM and TEM (Microscopy & Microanalysis 2017) 

Optimization of Automated Immuno EM for Both Pre- and Post-Embedding Labeling (Microscopy & Microanalysis 2018) 

Automated Rapid Preparation of Tissue Specimens for TEM Pathology (Microscopy & Microanalysis 2018) 

A Versatile All-in-One Automated Processor for Electron Microscopy. Microscopy & Microanalysis 2018)


Videos

Preparing Embedded TEM Specimens for Sectioning

This video tutorial shows how to trim and face an embedded block inside an mPrep/s capsule. The capsule is clamped into a microtome chuck for trimming, facing and sectioning.

1 minutes 17 seconds

Using the mPrep Workstation for Oriented and Unoriented Specimens

The tutorial starts by introducing the mPrep Workstation. Then it demonstrates how to load and orient specimens into mPrep capsules. Three methods are shown: pinch, compression and cup.

5 minutes 19 seconds

WEBINAR: Walk-away Biological Specimen Prep for the 21st Century Lab

This 18-minute webinar (broadcast 25 September 2019) presents how the ASP-1000 automates the preparation of biological specimens for TEM and SBEM (serial block face SEM) to provide walk-away convenience, reduced processing time, and easy clean-up. The webinar also discusses how the ASP-1000 provides pre-programmed protocols for preparing multiple tissues and for immunolabeling, and how users can easily modify existing or create new protocols.


Instructions

A single page graphical display of the three steps to insert specimens into mPrep/s capsules using the mPrep/s insertion tool. Use this guide as a lab reference poster.

A single page graphical display of the three steps to orient and load specimens into mPrep/s capsules. Both compression and pinch methods are shown. Use this guide as a lab reference poster.

Instructions for using mPrep/s capsules for specimen handling and processing.

Print this 96-well plate template to organize protocols into columns and rows with a microtiter 96-well plate.

This technical note introduces using mPrep capsule labeling for tracking specimens and grids with a data management system. Topics addressed include mPrep labels, 2D datamatrix barcodes, barcode readers and laboratory information management systems.


mPrep specimen products

mPrep/s™ Capsule Pack

8 capsules, 12 screens, storage box, labels

Product SKU: 22200

$48.00

mPrep/s™ Capsules – 96 count

96 capsules, screens & blank label sets

Product SKU: 22500

$576.00

mPrep/s™ Insertion Tool

Inserts, removes and adjusts screens in mPrep/s capsules

Product SKU: 32010

$25.00

mPrep/s™ Workstation

Prepare specimens and insert into mPrep/s capsules with or without orientation using integrated screen insertion tool

Product SKU: 42100

$950.00

mPrep/Bench™ model 96s

96-well silicone rack for mPrep capsules

Product SKU: 34000

$45.00

mPrep/f 30 Standard Filter Coupler™

16 couplers

Product SKU: 31500

$48.00

mPrep/f 13 Extreme Filter Coupler™

16 couplers

Product SKU: 31600

$48.00

mPrep™ CPD Holder

Holds up to 6 mPrep/s or mPrep/g capsules in Tousimis® CPD apparatus

Product SKU: 33010

$225.00

ASP-1000 mPrep Automatic Specimen Processor

Programable specimen and grid preparation.     Request a Quotation

Product SKU: ASP1000

Reagent Reservoirs

Chemically resistant 15ml reservoirs, 50 per pack

Product SKU: 52001

$22.00

Microwell Plates

Chemically resistant 96-well plates, 10 per pack

Product SKU: 51001

$39.00

mPrep/s™ Pipettor Kit eight-channel

eight-channel pipettor, mPrep/s capsule pack, 96 pipette tips

Product SKU: 43250

$686.00

mPrep/s™ Pipettor Kit single-channel

single-channel pipettor, mPrep/s capsule pack, 96 pipette tips

Product SKU: 43150

$366.00

Staining tissue sections on TEM grids

The mPrep System streamlines the entire tissue preparation and grid staining workflow.

Problems

Staining tissue sections on grids requires using fine forceps to transfer grids into grid boxes, onto stain droplets or staining apparatuses, back into grid boxes, and eventually onto the TEM stage. As a result, microscopists experience multiple challenges:

  • Grids are easily damaged
  • Handling grids is tedious
  • Grids are easily lost or mixed up
  • Difficulty achieving uniform staining times across multiple grids
  • Stains exposed to air can be contaminated, precipitate, and evaporate

 

mPrep solutions

  • Encapsulated grids reduce handling and damage.
  • Grids may only be directly handled at the microtome and the microscope
  • Grids in mPrep/g capsules are always labeled
  • Simultaneous processing assures uniform timing
  • Enclosed capsules reduce and can even eliminate air exposure

Learn more about staining tissue sections on TEM grids in the application notes below.

Heart (TEM)

Brain - Vampire bat (TEM)

Application Notes

Required

mPrep/g capsules to hold TEM grids

mPrep/f couplers to prevent introducing damaging reagents into pipettors

Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules

Recommended

Reagent reservoirs

Microwell plates

mPrep/Bench to seal capsule bottoms during long reagent treatments

Gilson Pipetman Neo (P200) pipettors bundled with mPrep/g capsules

mPrep/g Pipettor Kit single-channel

  • Single-channel pipettor
  • mPrep/s capsule pack
  • 96 pipette tips

mPrep/g Pipettor Kit eight-channel

  • Eight-channel pipettor
  • mPrep/g capsule pack
  • 96 pipette tips

Publications

Capsule-Based Processing and Handling of Electron Microscopy Specimens and Grids (Microscopy Today 2015) 

Improving the Reliability, Ease, and Efficiency of Section Staining in a Diagnostic Laboratory with the mPrep/g System for TEM Grid Processing (Microscopy & Microanalysis 2015) 

Automated, Programmable Processing of Specimens and Grids with the mPrep ASP-1000 (Microscopy & Microanalysis 2015) 

Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules (Microscopy & Microanalysis 2015) 

Reducing Reagent Consumption and Improving Efficiency of Specimen Fixation and Embedding, Grid Staining and Archiving using mPrep™ Capsule Processing (Microscopy & Microanalysis 2011) 


Videos

Specimen and Grid Reagent Processing for TEM and SEM

This video tutorial demonstrates how to use mPrep capsules with multi-channel pipettors and common lab ware to simultaneously deliver reagents to multiple specimens and grids. The video demonstrates TEM fixation and embedding. Reagent processing procedures are similar for all specimen types and for grids with mPrep capsules .

6 minutes 4 seconds


Instructions

Instructions for using mPrep/g capsules for staining sections on grids with uranyl acetate and lead citrate.

Instructions for using mPrep/g capsules for grid handling and processing.

This technical note introduces using mPrep capsule labeling for tracking specimens and grids with a data management system. Topics addressed include mPrep labels, 2D datamatrix barcodes, barcode readers and laboratory information management systems.

A graphical display illustrating how to load grids into mPrep/g capsules, and how to use pipettors to stain or label grids. Use this guide as a lab reference poster.


mPrep grid products

mPrep/Bench™ model 96s

96-well silicone rack for mPrep capsules

Product SKU: 34000

$45.00

mPrep/g™ Capsule Pack

16 capsules, storage box, labels

Product SKU: 21300

$48.00

mPrep/g™ Capsules – 96 count

96 capsules, 96 label sets

Product SKU: 21500

$288.00

mPrep/f 30 Standard Filter Coupler™

16 couplers

Product SKU: 31500

$48.00

mPrep/f 13 Extreme Filter Coupler™

16 couplers

Product SKU: 31600

$48.00

mPrep™ CPD Holder

Holds up to 6 mPrep/s or mPrep/g capsules in Tousimis® CPD apparatus

Product SKU: 33010

$225.00

ASP-1000 mPrep Automatic Specimen Processor

Programable specimen and grid preparation.     Request a Quotation

Product SKU: ASP1000

Reagent Reservoirs

Chemically resistant 15ml reservoirs, 50 per pack

Product SKU: 52001

$22.00

Microwell Plates

Chemically resistant 96-well plates, 10 per pack

Product SKU: 51001

$39.00

mPrep/g™ Pipettor Kit eight-channel

eight-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43200

$686.00

mPrep/g™ Pipettor Kit single-channel

single-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43100

$366.00

Negative staining of macromolecules

The mPrep system simplifies the preparation of macromolecular and particulate specimens for TEM.

Problems

Negative stain specimen preparation requires fine forceps to transfer filmed grids across droplets of macromolecule or particle suspensions, negative stains, and rinses.  This presents microscopists with multiple challenges:

  • Grids and support films are easily damaged
  • Handling grids is tedious
  • Grids are easily lost or mixed up
  • Limited quantities of specimen solutions may be available
  • Specimen and reagent solutions may evaporate, precipitate or be contaminated
  • Inconsistent adsorption and staining times across multiple grids

 

 mPrep  solutions

  • Encapsulated grids reduce handling and  damage
  • Grids with specimens may require direct handling only at the microscope
  • Grids in mPrep/g capsules are always labeled
  • Capsules require only 35 μl to prepare two grids
  • Enclosed capsules reduce or eliminate air exposure
  • Simultaneous processing assures uniform timing

DNA RecA (TEM negative stain)

Application Notes

This application note illustrates the preparation of macromolecular specimens for negative stain TEM using mPrep/g capsules, pipettors and Formvar®-filmed TEM grids. The example is a DNA-RecA complex, although the protocol is applicable to many other specimens. The specimen preparation and microscopy was performed in the Department of Biochemistry at the University of Wisconsin – Madison by Mr. Jay Campbell and Dr. Sindhu Chitteni-Pattu, in the laboratory of Dr. Michael M Cox.

Required

mPrep/g capsules to hold TEM grids

mPrep/f couplers to prevent introducing damaging reagents into pipettors

Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules

Recommended

Reagent reservoirs
Microwell plates
mPrep/bench to seal capsule bottoms during long reagent treatments

Gilson Pipetman Neo (P200) pipettors bundled with mPrep/g capsules.

mPrep/g Pipettor Kit single-channel

  • Single-channel pipettor
  • mPrep/s capsule pack
  • 96 pipette tips

mPrep/g Pipettor Kit eight-channel

  • Eight-channel pipettor
  • mPrep/g capsule pack
  • 96 pipette tips

Publications

Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules (Microscopy & Microanalysis 2015) 


Videos

Specimen and Grid Reagent Processing for TEM and SEM

This video tutorial demonstrates how to use mPrep capsules with multi-channel pipettors and common lab ware to simultaneously deliver reagents to multiple specimens and grids. The video demonstrates TEM fixation and embedding. Reagent processing procedures are similar for all specimen types and for grids with mPrep capsules .

6 minutes 4 seconds


Instructions

Instructions for using mPrep/g capsules for grid handling and processing.

Print this 96-well plate template to organize protocols into columns and rows with a microtiter 96-well plate.

A graphical display illustrating how to load grids into mPrep/g capsules, and how to use pipettors to stain or label grids. Use this guide as a lab reference poster.


mPrep grid products

mPrep/Bench™ model 96s

96-well silicone rack for mPrep capsules

Product SKU: 34000

$45.00

mPrep/g™ Capsule Pack

16 capsules, storage box, labels

Product SKU: 21300

$48.00

mPrep/g™ Capsules – 96 count

96 capsules, 96 label sets

Product SKU: 21500

$288.00

mPrep/f 30 Standard Filter Coupler™

16 couplers

Product SKU: 31500

$48.00

mPrep/f 13 Extreme Filter Coupler™

16 couplers

Product SKU: 31600

$48.00

mPrep™ CPD Holder

Holds up to 6 mPrep/s or mPrep/g capsules in Tousimis® CPD apparatus

Product SKU: 33010

$225.00

ASP-1000 mPrep Automatic Specimen Processor

Programable specimen and grid preparation.     Request a Quotation

Product SKU: ASP1000

Reagent Reservoirs

Chemically resistant 15ml reservoirs, 50 per pack

Product SKU: 52001

$22.00

Microwell Plates

Chemically resistant 96-well plates, 10 per pack

Product SKU: 51001

$39.00

mPrep/g™ Pipettor Kit eight-channel

eight-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43200

$686.00

mPrep/g™ Pipettor Kit single-channel

single-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43100

$366.00

Preparing viruses on TEM grids

The mPrep system facilitates preparation of viruses and other microorganisms for TEM examination, including  those prepared in Biological Safety Level 3 or 4 laboratory suites.

Problems

Virus sample preparation for TEM requires using fine forceps to transfer filmed grids throughout the process.  Grids boxes may require decontamination prior to transfer to the TEM.

  • Grids and support films are easily damaged
  • Grids are easily lost or mixed up
  • Sharp forceps are a puncture risk to protective clothing
  • Grid handling is especially difficult wearing BSL3/4 positive pressure suits
  • Limited quantities of specimen solutions may be available
  • Specimen and reagent solutions exposed to air may evaporate, precipitate or be contaminated
  • Grids and grid storage containers require decontamination
  • Adsorption and staining times may be inconsistent across multiple grids

 

mPrep solutions

 

  • Encapsulated grids reduce handling and damage
  • Grids in mPrep/g capsules are always labeled
  • Forceps are not used for virus preparation
  • Direct handling of grids may require handling only at the microscope
  • Capsules require only 35 μl to prepare two grids
  • Enclosed capsules reduce or eliminate air exposure
  • Grids in capsules are decontaminated as a unit
  • Simultaneous processing assures uniform timing

T6 Phage (negative stain)

Ebola virus (negative stain)

Application Notes

This application note shows the negative stain TEM preparation of pathogenic Ebola virus in high containment BSL4 labs. Scientists used mPrep/g capsules to eliminate grid handling in the BSL suite. This resulted in easier, safer processing and more reproducible sample preparation.  Specimen preparation and microscopy were performed by the US Army Medical Research Institute of Infectious Diseases (USAMRIID) Pathology Division, at Ft. Detrick, MD.

Required

mPrep/g capsules to hold TEM grids
mPrep/f 13 extreme couplers to prevent introducing damaging reagents or viruses into pipettors
Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules

Recommended

Reagent reservoirs

Microwell plates

mPrep/bench to seal capsule bottoms during long reagent treatments

Gilson Pipetman Neo (P200) pipettors bundled with mPrep/g capsules.

mPrep/g Pipettor Kit single-channel

  • Single-channel pipettor
  • mPrep/s capsule pack
  • 96 pipette tips

mPrep/g Pipettor Kit eight-channel

  • Eight-channel pipettor
  • mPrep/g capsule pack
  • 96 pipette tips

Publications

Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules (Microscopy & Microanalysis 2015) 

Scanning Transmission Electron Microscopy (STEM) for Accurate Virus Quantification (Defense Technical Information Center 2018)

Improved Virus Specimen Preparation for Transmission Electron Microscopy using mPrep/g Capsules: Applications in BSL3-4 Laboratories (Ft. Detrick Science Fair 2016)

Improved Virus Specimen Preparation for Transmission Electron Microscopy using mPrep/g Capsules: Applications in BSL3-4 Laboratories (Microscopy & Microanalysis 2016) 

Using Scanning Transmission Electron Microscopy (STEM) for Accurate Virus Dosing Quantification (Microscopy & Microanalysis 2016) 

Preparation of viral samples within biocontainment for ultrastructural analysis: Utilization of an innovative processing capsule for negative staining (J Virological Methods 2016)

Accurate virus quantitation using a Scanning Transmission Electron Microscopy (STEM) detector in a scanning electron microscope (J. Virological Methods 2017)

Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment (J Visualized Experiments 2017) 



Instructions

Instructions for using mPrep/g capsules for grid handling and processing.

Print this 96-well plate template to organize protocols into columns and rows with a microtiter 96-well plate.

A graphical display illustrating how to load grids into mPrep/g capsules, and how to use pipettors to stain or label grids. Use this guide as a lab reference poster.

A graphical display that illustrates how to easily prepare virus specimens for negative stain TEM in Biological Safety Level 3-4, and other laboratories. Use this guide as a lab reference poster.


mPrep grid products

mPrep/Bench™ model 96s

96-well silicone rack for mPrep capsules

Product SKU: 34000

$45.00

mPrep/g™ Capsule Pack

16 capsules, storage box, labels

Product SKU: 21300

$48.00

mPrep/g™ Capsules – 96 count

96 capsules, 96 label sets

Product SKU: 21500

$288.00

mPrep/f 30 Standard Filter Coupler™

16 couplers

Product SKU: 31500

$48.00

mPrep/f 13 Extreme Filter Coupler™

16 couplers

Product SKU: 31600

$48.00

mPrep™ CPD Holder

Holds up to 6 mPrep/s or mPrep/g capsules in Tousimis® CPD apparatus

Product SKU: 33010

$225.00

ASP-1000 mPrep Automatic Specimen Processor

Programable specimen and grid preparation.     Request a Quotation

Product SKU: ASP1000

Reagent Reservoirs

Chemically resistant 15ml reservoirs, 50 per pack

Product SKU: 52001

$22.00

Microwell Plates

Chemically resistant 96-well plates, 10 per pack

Product SKU: 51001

$39.00

mPrep/g™ Pipettor Kit eight-channel

eight-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43200

$686.00

mPrep/g™ Pipettor Kit single-channel

single-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43100

$366.00

On Grid Immunogold labeling (IGL)

Immunogold labeling (IGL) or immunolabeling is a powerful methodology for localizing structures in biological specimens.

Problems

Immunolabeling tissue sections on TEM grids using “droplet” methods requires the timed transfer of multiple grids through a dozen reagents in a process that can take hours or even a full day. As a result, microscopists experience multiple challenges:

  • Difficultly achieving consistent reagent timing, which limits experimental reproducibility
  • Grids are easily damaged, lost, or mixed up
  • IGL reagents exposed to air can evaporate, precipitate or become contaminated
  • IGL reagents and antibodies are expensive

 

mPrep solutions

  • The mPrep System addresses these challenges:
    • Simultaneous processing assures identical reagent timing and reproducible results
    • Encapsulated grids are identified and protected from loss or damage
    • Enclosed capsules limit air exposure
    • mPrep/g capsules require only 20 ul per grid

 

Automated IGL with the mPrep ASP-1000

With the ASP-1000 the entire immunolabeling process is automated. Simply set up the reagents, start the program, and walk away for the rest of the day or overnight. Use with mPrep/g capsules for grids, and mPrep/s capsules for tissue en bloc IGL.

 

Manual IGL with a multi-channel pipettor

Using a multichannel pipettor ensures that reagent incubation timing is identical for all grids. Because grids are safely encapsulated reagent exchanges are fast and easy. Use with mPrep/s capsules for tissue en bloc IGL.

6 nm IGL, endosome complex, C. elegans

6 nm IGL, endosome complex, C. elegans

Application Notes

This brief technical note illustrates automation of immunogold labeling using the mPrep ASP-1000 automated specimen processor. Researchers at the University of Wisconsin prepared C. elegans tissue sections on TEM grids and then used the ASP-1000 to immunolabel an endosomal sorting component with 6 nm gold. The entire 7.5 hour immunolabeling protocol was automatically accomplished.  This note also illustrates automated application of 10 nm gold fiducial markers onto thick sections for tomography.

Required

mPrep/g capsules to hold TEM grids
mPrep/f couplers to prevent introducing reagents into the ASP-1000 or pipettors

ASP-1000 for automated immunogold labeling

Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules

mPrep/s capsules to hold tissue samples for en bloc IGL

Recommended

Microwell plates to hold reagents

Foil seals to cover reagents until use

Example: 1.5 mil thick  pierceable AlumaSeal II Film for PCR plates (Contact us for more information)

Publications

Fully-automated Immunogold Labeling of Resin Embedded Specimens and On-grid Deposition of Gold Fiducial Particles (Microscopy & Microanalysis 2016) 

Preparing LR White Embedded Tissue with mPrep/s Specimen Capsules (Microscopy & Microanalysis 2017) 

Optimization of Automated Immuno EM for Both Pre- and Post-Embedding Labeling (Microscopy & Microanalysis 2018) 

Neuraminidase 1-mediated desialylation of the mucin 1 ectodomain releases a decoy receptor that protects against Pseudomonas aeruginosa lung infection (J Biological Chemistry 2019)


Videos

WEBINAR: Walk-away Biological Specimen Prep for the 21st Century Lab

This 18-minute webinar (broadcast 25 September 2019) presents how the ASP-1000 automates the preparation of biological specimens for TEM and SBEM (serial block face SEM) to provide walk-away convenience, reduced processing time, and easy clean-up. The webinar also discusses how the ASP-1000 provides pre-programmed protocols for preparing multiple tissues and for immunolabeling, and how users can easily modify existing or create new protocols.


Instructions

Instructions for using mPrep/g capsules for grid handling and processing.

A graphical display illustrating how to load grids into mPrep/g capsules, and how to use pipettors to stain or label grids. Use this guide as a lab reference poster.

Protocol for immuno-labeling grids with mPrep/g


mPrep grid products

mPrep/Bench™ model 96s

96-well silicone rack for mPrep capsules

Product SKU: 34000

$45.00

mPrep/g™ Capsule Pack

16 capsules, storage box, labels

Product SKU: 21300

$48.00

mPrep/g™ Capsules – 96 count

96 capsules, 96 label sets

Product SKU: 21500

$288.00

mPrep/f 30 Standard Filter Coupler™

16 couplers

Product SKU: 31500

$48.00

mPrep/f 13 Extreme Filter Coupler™

16 couplers

Product SKU: 31600

$48.00

mPrep™ CPD Holder

Holds up to 6 mPrep/s or mPrep/g capsules in Tousimis® CPD apparatus

Product SKU: 33010

$225.00

ASP-1000 mPrep Automatic Specimen Processor

Programable specimen and grid preparation.     Request a Quotation

Product SKU: ASP1000

Reagent Reservoirs

Chemically resistant 15ml reservoirs, 50 per pack

Product SKU: 52001

$22.00

Microwell Plates

Chemically resistant 96-well plates, 10 per pack

Product SKU: 51001

$39.00

mPrep/g™ Pipettor Kit eight-channel

eight-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43200

$686.00

mPrep/g™ Pipettor Kit single-channel

single-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43100

$366.00

Colloidal gold fiducial markers for 3-D Tomography

Accurate image alignment is essential for 3-D image reconstruction in TEM tomography. Colloidal gold particles are used as fiducial markers to align the different tilt angle images of thick sections.

Problems

For successful image reconstruction colloidal gold fiducial markers must be distributed uniformly over both sides of the grid. The common protocol is to apply a droplet of a colloidal gold suspension to each grid, and then wick off the droplet after a short adsorption time. The result is that microscopists experience multiple challenges:

  • Inconsistent results from grid to grid
  • Uneven particle distributions on each grid and each grid side
  • Time consuming to prepare multiple grids
  • Grids are easily damaged, lost, or mixed up

 

mPrep solutions

The mPrep System addresses these challenges:

  • Consistent results on every grid
  • Uniform particle distribution on both grid sides
  • Automated or manual simultaneous preparation of up to 16 grids
  • Encapsulated grids are protected from damage or loss, and are always identified

 

Automated with the mPrep ASP-1000

Application of colloidal gold fiducial particles onto grids is fully automated with the mPrep ASP-1000. Colloidal gold particles are applied simultaneously to both sides of up to 16 grids. Automated agitation ensures consistent results.

 

Manual IGL with a multi-channel pipettor

Using mPrep/g capsules with an 8-channel pipettor, colloidal gold fiducial particles are applied simultaneously to both sides of up to 16 grids. With a multichannel pipettor, manual agitation ensures consistent results for multiple grids prepared simultaneously.

10 nm Au fiducial particle, 250 nm section

10 nm Au fiducial particle, 250 nm section

Required

mPrep/g capsules to hold TEM grids

mPrep/f couplers to prevent introducing reagents into the ASP-1000 or pipettors

ASP-1000 for automated immunogold labeling

Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules

Recommended

Microwell plates to hold reagents

Product Descriptions

Publications

Fully-automated Immunogold Labeling of Resin Embedded Specimens and On-grid Deposition of Gold Fiducial Particles (Microscopy & Microanalysis 2016) 



Instructions

Instructions for using mPrep/g capsules for grid handling and processing.

A graphical display illustrating how to load grids into mPrep/g capsules, and how to use pipettors to stain or label grids. Use this guide as a lab reference poster.

Protocol for immuno-labeling grids with mPrep/g


Product Descriptions

mPrep grid products

mPrep/Bench™ model 96s

96-well silicone rack for mPrep capsules

Product SKU: 34000

$45.00

mPrep/g™ Capsule Pack

16 capsules, storage box, labels

Product SKU: 21300

$48.00

mPrep/g™ Capsules – 96 count

96 capsules, 96 label sets

Product SKU: 21500

$288.00

mPrep/f 30 Standard Filter Coupler™

16 couplers

Product SKU: 31500

$48.00

mPrep/f 13 Extreme Filter Coupler™

16 couplers

Product SKU: 31600

$48.00

mPrep™ CPD Holder

Holds up to 6 mPrep/s or mPrep/g capsules in Tousimis® CPD apparatus

Product SKU: 33010

$225.00

ASP-1000 mPrep Automatic Specimen Processor

Programable specimen and grid preparation.     Request a Quotation

Product SKU: ASP1000

Reagent Reservoirs

Chemically resistant 15ml reservoirs, 50 per pack

Product SKU: 52001

$22.00

Microwell Plates

Chemically resistant 96-well plates, 10 per pack

Product SKU: 51001

$39.00

mPrep/g™ Pipettor Kit eight-channel

eight-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43200

$686.00

mPrep/g™ Pipettor Kit single-channel

single-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43100

$366.00

Preparing nanoparticles on TEM grids

The mPrep system simplifies the preparation of nanoparticles for TEM.

Problems

Nanoparticle specimen prep requires  forceps to transfer filmed grids through multiple reagents. Grids are stored in grid boxes until TEM imaging.

  • Grids and support films are easily damaged
  • Grids are easily lost or mixed up
  • Limited quantities of specimen solutions may be available
  • Specimen and reagent solutions may evaporate, precipitate or be contaminated
  • Adsorption and staining times may be inconsistent across multiple grids

 

mPrep solutions

  • Encapsulated grids reduce handling and damage
  • Grids in mPrep/g capsules are always labeled
  • Capsules require only 35 μl to prepare two grids
  • Enclosed capsules reduce or eliminate air exposure
  • Simultaneous processing assures uniform timing

Nanoparticles (TEM negative stain)

Application Notes

This illustrated protocol shows the preparation of nanoparticle specimens for negative stain TEM using Formvar®-filmed grids in mPrep/g capsules. Eight different formulations of ~100 nm drug delivery particles were prepared simultaneously for a pharmaceutical company to evaluate structure and uniformity. This general sample preparation method can be applied to other nano-scale specimens including nano-fibers and viruses.

This application note illustrates the preparation of macromolecular specimens for negative stain TEM using mPrep/g capsules, pipettors and Formvar®-filmed TEM grids. The example is a DNA-RecA complex, although the protocol is applicable to many other specimens. The specimen preparation and microscopy was performed in the Department of Biochemistry at the University of Wisconsin – Madison by Mr. Jay Campbell and Dr. Sindhu Chitteni-Pattu, in the laboratory of Dr. Michael M Cox.

Required

mPrep/g capsules to hold TEM grids
mPrep/f couplers to prevent introducing damaging reagents into pipettors
Pipettor (such as a Gilson P200 volume) to enable reagent pipetting into mPrep/s capsules

Recommended

Reagent reservoirs

Microwell plates

mPrep/bench to seal capsule bottoms during long reagent treatments

Gilson Pipetman Neo (P200) pipettors bundled with mPrep/g capsules

mPrep/g Pipettor Kit single-channel

  • Single-channel pipettor
  • mPrep/s capsule pack
  • 96 pipette tips

mPrep/g Pipettor Kit eight-channel

  • Eight-channel pipettor
  • mPrep/g capsule pack
  • 96 pipette tips

Publications

Nano-Particle TEM Sample Preparation Primer (Microscopy & Microanalysis 2016)

Efficient and Documented Preparation of Pharmaceutical Particles for Correlative Microscopy Analyses using mPrep Capsule Processing (Microscopy & Microanalysis 2015) 

Synthesis of nano-Pt onto ceria support as catalyst for water–gas shift reaction by Reactive Spray Deposition Technology (Applied Catalysis A: General 2014)

Comparative study for low temperature water-gas shift reaction on Pt/ceria catalysts: Role of different ceria supports (Applied Catalysis A: General 2015)



Instructions

Instructions for using mPrep/g capsules for grid handling and processing.

Instructions for using mPrep/g capsules for staining sections on grids with uranyl acetate and lead citrate.

A graphical display illustrating how to load grids into mPrep/g capsules, and how to use pipettors to stain or label grids. Use this guide as a lab reference poster.


mPrep grid products

mPrep/Bench™ model 96s

96-well silicone rack for mPrep capsules

Product SKU: 34000

$45.00

mPrep/g™ Capsule Pack

16 capsules, storage box, labels

Product SKU: 21300

$48.00

mPrep/g™ Capsules – 96 count

96 capsules, 96 label sets

Product SKU: 21500

$288.00

mPrep/f 30 Standard Filter Coupler™

16 couplers

Product SKU: 31500

$48.00

mPrep/f 13 Extreme Filter Coupler™

16 couplers

Product SKU: 31600

$48.00

mPrep™ CPD Holder

Holds up to 6 mPrep/s or mPrep/g capsules in Tousimis® CPD apparatus

Product SKU: 33010

$225.00

ASP-1000 mPrep Automatic Specimen Processor

Programable specimen and grid preparation.     Request a Quotation

Product SKU: ASP1000

Reagent Reservoirs

Chemically resistant 15ml reservoirs, 50 per pack

Product SKU: 52001

$22.00

Microwell Plates

Chemically resistant 96-well plates, 10 per pack

Product SKU: 51001

$39.00

mPrep/g™ Pipettor Kit eight-channel

eight-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43200

$686.00

mPrep/g™ Pipettor Kit single-channel

single-channel pipettor, mPrep/g capsule pack, 96 pipette tips

Product SKU: 43100

$366.00